Research Paper Volume 16, Issue 19 pp 12909—12927

Figure 1. MIAT is upregulated in fibrotic buccal tissues and primary fibrotic buccal mucosa fibroblasts from patients with OSF. (A) RNA-sequencing analysis showed that MIAT was an up-regulated differentially expressed gene (fold change ≥ 2.0; p < 0.01) in fibrotic tissue samples (OSF) compared to normal tissues (N) from patients with OSF (n=2) and healthy individuals (n=2). (B) The relative expression of MIAT in samples of normal (N; n=25) and fibrotic (OSF; n=25) tissue was assessed by qRT-PCR analysis. Data are mean ± S.D. (C) The relative expression of MIAT was further assessed in primary normal buccal mucosa fibroblasts (nBMFs; n=5) and fibrotic buccal mucosa fibroblasts (fBMFs; n=5) by qRT-PCR, with differences between groups analyzed using the paired Student’s t-test. (D–F) A significant positive correlation was observed between the expression of MIAT and fibrotic markers, including ACTA2 (encoding α-SMA; D), COL1A1 (E), and FN1 (F) in samples of fibrotic tissue (n=40).