Research Paper Volume 16, Issue 18 pp 12651—12666
Unveiling senescence-associated secretory phenotype in epidermal aging: insights from reversibly immortalized keratinocytes
Figure 5. Organotypic 3D skin model constructed with reversibly immortalized NHEK showed aging phenotypes with Dox induction. (A) Flowchart of organotypic skin culture. (B) H&E staining, SA-β-GAL staining, and immunofluorescent staining of Loricrin and K14 in organotypic skin cultures constructed with reversibly immortalized keratinocytes with PBS or Dox treatment. Scale bar = 20 μm. (C) IHC staining analysis of SASP-related proteins VEGFA, IGFBP3 and MMP1 in organotypic 3D skin cultures constructed with reversibly immortalized keratinocytes with either PBS or Dox treatment. Scale bar = 20 μm.