Research Paper Volume 16, Issue 18 pp 12651—12666
Unveiling senescence-associated secretory phenotype in epidermal aging: insights from reversibly immortalized keratinocytes
Figure 4. Gene set enrichment analysis revealed enrichment of SASP-related genes set in the DEGs. (A) Gene expression heatmap of SASP-related genes in reversibly immortalized NHEK with PBS or Dox treatment. (B) GSEA results of SASP gene set. (C) Volcano plot of up- or downregulated genes in reversibly immortalized NHEK with PBS or Dox treatment with significant genes in SASP gene set annotated. (D) Protein levels of VEGFA, SERPINB2 and MMP1 were tested by Western blot in reversibly immortalized keratinocytes with PBS or Dox treatment. (E) mRNA levels of VEGFA, SERPINB2 and MMP1 were tested by qPCR in reversibly immortalized keratinocytes with PBS or Dox treatment.