Research Paper Volume 16, Issue 19 pp 12866—12892
DNA methylation-driven gene FAM3D promotes colorectal cancer growth via the ATF4-SESN2-mTORC1 pathway
Figure 6. FAM3D functions in CRC cells by targeting SESN2 and activating the mTORC1 pathway. (A) The mRNA and protein levels of SESN2 in FAM3D-knockout LoVo cells with SESN2 knockdown. (B) The mRNA and protein levels of SESN2 in FAM3D-overexpressing LoVo cells with SESN2 overexpression. (C) Colony formation assay of FAM3D-knockout LoVo cells with SESN2 knockdown. (D) Colony formation assay of FAM3D-overexpressing LoVo cells with SESN2 overexpression. (E) CCK-8 assay of FAM3D-knockout LoVo cells with SESN2 knockdown. (F) CCK-8 assay of FAM3D-overexpressing LoVo cells with SESN2 overexpression. (G) A wound healing assay of FAM3D-knockout LoVo cells with SESN2 knockdown. (H) A wound healing assay of FAM3D-overexpressing LoVo cells with SESN2 overexpression. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.