Research Paper Volume 16, Issue 19 pp 12850—12865

Figure 3. USP4 promotes the proliferation of PTC. (A) Western blot analysis to determine the knockdown efficiency of USP4 in B-CPAP and TPC-1 cells; (B) qPCR analysis to determine the knockdown efficiency of USP4 in B-CPAP and TPC-1 cells; (C, D) CCK-8 assay to assess the proliferative capacity of B-CPAP and TPC-1 cells following USP4 knockdown; (E) Representative images from colony formation assays of B-CPAP and TPC-1 cells with USP4 knockdown; (F) Statistical quantification of colony numbers in B-CPAP and TPC-1 cells with USP4 knockdown; (G, H) Western blot analysis to determine USP4 overexpression in K1 and KTC-1 cells; (I, J) CCK-8 assay to assess the proliferative capacity of K1 and KTC-1 cells expressing USP4; (K) Representative images from colony formation assays of K1 and KTC-1 cells expressing USP4; (L) Statistical quantification of colony numbers in K1 and KTC-1 cells expressing USP4. All ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.