Figure 4. Functional exploration of GJA1 at the single-gene level. (A) Immunohistochemistry results demonstrating the expression of GJA1 in older populations. (B) Immunofluorescence demonstrating the enrichment of mouse brain tissue for GJA1 in the cortex. (C) GO analysis of GJA1. (D) KEGG analysis of GJA1. (E) Immune-related function analysis of GJA1. (F) Percentage of immune cells in the immune cell ratio between groups with high and low expression of GJA1 in Temporal Cortex. (G) Lymphocyte subpopulation occupancy in the two groups. (H, I) Correlation between lymphocyte subpopulation and GJA1 in the two groups.