Research Paper Volume 16, Issue 18 pp 12543—12558
DDIT3 switches osteogenic potential of BMP9 to lipogenic by attenuating Wnt/β-catenin signaling via up-regulating DKK1 in mesenchymal stem cells
Figure 7. Effects of DKK1 knockdown and/or DDIT3 on BMP9-induced osteogenic and adipogenic markers in C3H10T1/2 cells. (A) Real-time PCR assay shows the effect of DDIT3 and/or DKK1 knockdown on Runx2 mRNA expression induced by BMP9. (B) Western blot results show the effect of DDIT3 and/or DKK1 knockdown on Runx2 protein level induced by BMP9. (C) Quantitative results of Western blot assay show the effect of DDIT3 and/or DKK1 knockdown on the Runx2 protein level induced by BMP9. (D) Real-time PCR assay shows the effect of DDIT3 and/or DKK1 knockdown on the mRNA expression of OPN induced by BMP9. (E) Western blot assay shows the effect of DDIT3 and/or DKK1 knockdown on the OPN protein level induced by BMP9. (F) Quantitative results of Western blot assay show the effect of DDIT3 and/or DKK1 knockdown on the OPN protein level induced by BMP9. (G) Real-time PCR assay results show the effect of DDIT3 and/or DKK1 knockdown on C/EBPα mRNA expression induced by BMP9. (H) Western blot assay shows the effect of DDIT3 and/or DKK1 knockdown on the C/EBPα protein level induced by BMP9. (I) Quantitative results of Western blot assay show the effect of DDIT3 and/or DKK1 knockdown on C/EBPα protein level induced by BMP9. “*” p <0.05, “**” p <0.01, compared with the control group; “#” p <0.05, “##” p<0.01, compared with the BMP9 group; “^” p <0.05, “^^” p<0.01, compared with DDIT3 and BMP9 group. All data were repeated in three independent experiments.