Research Paper Volume 16, Issue 18 pp 12543—12558
DDIT3 switches osteogenic potential of BMP9 to lipogenic by attenuating Wnt/β-catenin signaling via up-regulating DKK1 in mesenchymal stem cells
Figure 6. Effects of DDIT3 and/or BMP9 on DKK1 expression in C3H10T1/2 cells. (A) Western blot assay results show the DDIT3 effect on the protein level of DKK1 induced by BMP9. (B) Quantitative results of Western blot assay show the DDIT3 effect on the protein level of DKK1 induced by BMP9. (C) Western blot assay results show the protein level of DKK1 induced by BMP9 was reduced by DDIT3 knockdown. (D) Quantitative results of Western blot assay show the DDIT3 effect on DKK1 induced by BMP9. “*” P <0.05, “**” p <0.01, compared with the control group; “#” p <0.05, “##” p<0.01, compared with the BMP9 group. All data were repeated in three independent experiments.