Research Paper Volume 16, Issue 18 pp 12543—12558
DDIT3 switches osteogenic potential of BMP9 to lipogenic by attenuating Wnt/β-catenin signaling via up-regulating DKK1 in mesenchymal stem cells
Figure 4. Effects of DDIT3 on BMP9-induced adipogenic markers in C3H10T1/2 cells. (A) Real-time PCR results show the effect of DDIT3 on the mRNA expression of C/EBPα induced by BMP9. (B) Western blot assay results show the effect of DDIT3 on the protein level of C/EBPα induced by BMP9. (C) Quantitative results of Western blot assay show the effect of DDIT3 on the protein level of C/EBPα induced by BMP9. (D) Oil red O staining results show the effect of DDIT3 on lipid droplet formation induced by BMP9 on day 9 and day 11. (E) Real-time PCR assay results show the effect of DDIT3 knockdown on the mRNA level of C/EBPα induced by BMP9. (F) Western blot assay results show the effect of DDIT3 knockdown on the protein level of C/EBPα induced by BMP9. (G) Quantitative results of Western blot assay show the effect of DDIT3 knockdown on the protein level of C/EBPα induced by BMP9. (H) Oil red O staining results show the effect of DDIT3 knockdown on lipid droplet formation induced by BMP9 on day 9 and day 11. Compared with the BMP9 group, “*” p <0.05, “**” p <0.01; “#” p <0.05, “##” p<0.01. All data were repeated at three independent experiments.