Research Paper Volume 16, Issue 18 pp 12543—12558

Figure 3. Effects of DDIT3 knockdown on BMP9-induced osteogenic markers in C3H10T1/2 cells. (A) Real-time PCR results show the effect of DDIT3 knockdown on the mRNA level of Runx2 induced by BMP9. (B) Western blot assay results show the effect of DDIT3 knockdown on the protein level of Runx2 induced by BMP9. (C) Quantitative results of Western blot show the effect of DDIT3 knockdown on the protein level of Runx2 induced by BMP9. (D) ALP staining results show the effect of DDIT3 knockdown on the ALP activity induced by BMP9 on day 5 or day 7. (E) Quantitative results of ALP staining show the effect of DDIT3 knockdown on the ALP activity induced by BMP9 on day 5 or day 7. (F) Real-time PCR results show the effect of DDIT3 knockdown on the mRNA expression of OPN induced by BMP9 on day 9 and day 11. (G) Western blot results show the effect DDIT3 knockdown on the protein level of OPN induced by BMP9 on day 9 and day 11. (H) Quantitative results of Western blot assay show the effect DDIT3 knockdown on the protein level of OPN induced by BMP9 on day 9 and day 11. (I) Alizarin red S staining results show the effect of DDIT3 on matrix mineralization induced by BMP9 on day 18 and day 21. “*” p <0.05, “**” p <0.01; compared with the BMP9 group, “#” p <0.05, “##” p<0.01. All data were repeated in three independent experiments.