Research Paper Volume 16, Issue 18 pp 12543—12558
DDIT3 switches osteogenic potential of BMP9 to lipogenic by attenuating Wnt/β-catenin signaling via up-regulating DKK1 in mesenchymal stem cells
Figure 1. Effects of BMP9 on the expression of DDIT3 in C3H10T1/2 cells. (A) Real-time PCR assay shows the expression of DDIT3 in several progenitors and MG63 cells. (B) Real-time PCR assay shows the effect of BMP9 on the mRNA expression level of DDIT3. (C) Western blot assay shows the effect of BMP9 on the protein level of DDIT3. (D) Quantitative analyses of Western blot assay show the effect of BMP9 on the protein level of DDIT3. (E, F) Real-time PCR and Western blot assay show the effect of DDIT3 recombinant adenovirus on the mRNA and protein level of DDIT3. (G) Quantitative analyses of Western blot assay show the effect of DDIT3 recombinant adenovirus on the protein level of DDIT3. (H–J) Real-time PCR and Western blot assay show the effect of DDIT3 siRNAs recombinant adenovirus on the mRNA and protein level of DDIT3. (K) Images show the infection of AdDDIT3 and AdsiDDIT3 in C3H10T1/2 cells. “**” p <0.01; Compared with the BMP9 group. All results were repeated in three independent experiments.