Research Paper Volume 16, Issue 21 pp 13181—13200

The effects of young and aged, male and female megakaryocyte conditioned media on angiogenic properties of endothelial cells

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Figure 7. Representative image of BMEC vessel-like formation assay. Images were taken at 10x magnification. Meshes (red outline) and nodes (red star) were automatically analyzed. Meshes were defined as areas enclosed by three or more nodes and nodes were defined as a point where two or more vessels intersect. A blinded analyzer manually measured the number of vessel-like structures (indicated by blue arrows) as well as vessel length.