Research Paper Volume 16, Issue 21 pp 13181—13200

Figure 5. Altered two-dimensional motility of BMECs with MK CM treatments. The changes in relative wound density (A-males, B-females) and wound width (C-males, D-females) were quantified through data collected every 2 hours over a 48-hour period. Significance was determined using a two-way ANOVA with Dunnett’s post-hoc test (significance bars are noted to the right on all panels). Cross-sectional analyses were made at 12-, 24-, and 48-hour timepoints for all panels as well. No significant differences in relative wound density (A, B) were observed in any cross-sectional analyses. A one-way ANOVA found that female BMEC wound closure (panel D) was significantly inhibited when treated with female aged MK CM at the 48-hour timepoint (p = 0.029), but all other cross-sectional analyses were not significant in panels C and D. Solid, dashed, and dotted lines represent the means (n = 3–16 biological replicates/group).