Research Paper Volume 16, Issue 21 pp 13181—13200
The effects of young and aged, male and female megakaryocyte conditioned media on angiogenic properties of endothelial cells
Figure 4. Aged female and male MK CM increase unidimensional, transwell migration of BMECs. The number of migrated male BMECs (A) and female BMECs (B) was quantified following an overnight incubation period. Data are expressed as a mean ± SD fold change relative to their respective controls (n = 4–10 biological replicates/group). Significance was determined using a one-way ANOVA with Tukey’s post-hoc test. Aged MK CM significantly increased the migration of BMECs compared to control regardless of sex. Representative images of male BMECs using crystal violet staining (C- Control, D- Young Male MK CM, E- Old Male MK CM, F- 5% FBS control). Representative images of female BMECs using crystal violet staining (G- Control, H- Young Female MK CM, I- Old Female MK CM, J- 5% FBS control).