Research Paper Volume 16, Issue 21 pp 13181—13200

Figure 3. Young and old male MK CM significantly increase female BMEC vessel-like structure length. The number of vessel-like structures formed and total length of vessel-like structures were quantified following an 8-hour incubation period for female BMECs treated with male MK CM (A, B) and male BMECs treated with female MK CM (C, D). Representative images of BMEC vessel-like formation assays for Control media (E, H), young MK CM (F, I), and old MK CM (G, J), treatment groups are also shown for both female BMECs treated with male MK CM (E–G) and male BMECs treated with female MK CM (H–J). Data are expressed as a mean ± SD fold change relative to their respective controls (n = 4–8 biological replicates/group). Significance was determined using one-way ANOVA with Tukey’s post-hoc analysis (B–D) or Kruskal-Wallis test with Dunn’s post-hoc analysis (A) depending on the normalcy of the data distribution as determined by a Shapiro-Wilk test.