Research Paper Volume 16, Issue 15 pp 11729—11743
Hsa-miR-181a-2-3p inhibits the oncogenicity of colon cancer by directly targeting STING
Figure 3. miR-181a-2-3p inhibits colon cancer in vivo and targets STING. (A) A nude mouse tumorigenic assay was used to assess the in vivo tumorigenic ability of colon cancer. (B) Weight of isolated tumors. (C) The volume change of the tumor. (D) A dual luciferase assay to measure miR-181a-2-3p and STING 3′-UTR regions. Note: The Y-axis is relative luciferase activity or expression, the ratio of Firefly Luciferase (luc2) and Renilla Luciferase (hRluc) to values in Wells of the same sample represents the relative luciferase expression. (E) STING expression was analyzed in colon cancer tissues based on the TCGA database. (F) Western blot analysis of STING expression in colon cancer cell lines overexpressing miR-181a-2-3p. Note: OV-miR-181a-2-3p is miR-181a-2-3p overexpression group. Ctrl is the control.