Research Paper Volume 16, Issue 15 pp 11668—11682

Figure 4. Effects of Solamargine on fluorescence relative expression of HBV core promoter and its series of mutants. (A) Construction of HBV pgpromoter and ENII/BCP fluorescence reporter vector plasmid by PCR; (B) Construct plasmid of ENII/BCP and its series of gene deletion mutants pGL4.74-HBenIIcp-Ce-del1 to pGL4.74-HBenIIcp-Ce-del10 by PCR; (C, D) Each mutant plasmid was transfected into Huh7 cells, and after 2 d Solamargine (5 μM) was added, and the samples were collected after 1 d culture. HBV pgpromoter and ENII/BCP fluorescence relative expression were detected by sea kidney luciferase assay (C). The fluorescence relative expression levels of ENII/BCP and its gene deletion mutants were also detected (D). The relative expression level of fluorescence in control group was 1. The results are means±SD of 3 independent experiments. Statistical differences compared with the control are shown. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, Student’s t-test.