Research Paper Volume 16, Issue 15 pp 11668—11682
Solamargine acts as an antiviral by interacting to MZF1 and targeting the core promoter of the hepatitis B virus gene
Figure 1. Effects of Solamargine and other natural compounds on the relative expression of HBV pgRNA. (A) Left: HepG2.2.15 cell model commonly used for in vitro studies of HBV; right: pEB-puromycin HBV genome stable expression cell line model constructed in this experiment. (B) Huh7 stable cell lines were fused to 90% and then placed on the plates. Afte 1d, natural compounds (5 μM) were added to the experimental groups. After 3d, the cells were collected and total RNA was extracted. The expression level of HBV pgRNA in the cells was detected by RT-qPCR. The quantitative expression level of HBV pgRNA in control group was 1. (C) Solamargine molecular structure. The results are means ± SD of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test.