Research Paper Volume 16, Issue 16 pp 11843—11856
LncRNA HAGLR regulates gastric cancer progression by regulating the miR-20a-5p/E2F1 axis
Figure 5. E2F1 is the downstream gene of miR-20a-5p. (A) The downstream targets of miR-20a-5p predicted by the miRDB and TargetScan databases. (B) The expression of E2F1 in GC tissues. (C) Correlations of the expression of HAGLR and E2F1. (D) Correlations of the expression of E2F1and miR-20a-5p. (E) The expression of miR-20a-5p in different cell lines. (F) The influence of miR-20a-5p overexpression on E2F1 mRNA and protein. (G) The influence of miR-20a-5p knockdown on E2F1 mRNA and protein. (H) Dual luciferase assay showed that miR-20a-5p downregulated E2F1. (I) Fluorescence in situ hybridization results. (J) Diagram of RIP experimental results. (*P < 0.05, **P < 0.01, ***P < 0.001).