Research Paper Volume 16, Issue 14 pp 11409—11433
Discovery of differentially expressed proteins for CAR-T therapy of ovarian cancers with a bioinformatics analysis
Figure 6. Research stages and workflow. The four research red lines are differential expression analysis, differentially expressed genes (DEGs), protein localization analysis, oncogenicity analysis, and pathway and gene ontology analyses. Therefore, in this study, we carried out multilevel screening to reduce the potential for errors or discrepancies later. What we mean by multilevel screening is first looking at the significance of the expression of a gene in normal and cancer samples from the dataset, then checking the significance of the significant gene again in a different database to ensure that the gene is significantly expressed only in cancer cells.