Research Paper Volume 16, Issue 15 pp 11553—11567
The deubiquitinating enzymes-related signature predicts the prognosis and immunotherapy response in breast cancer
Figure 6. Construction of the OTUB2 knockdown cell and the functional experiment. (A) OTUB2 was successfully knocked down in MCF-7 and YCCB1 cells using lentiviral transfection method. (B) The CCK8 experiment showed that the number of OTUB2 knockdown cells decreased compared to sh-NC cells at various time points. (C) The clone formation experiment suggests that OTUB2 knockdown cells may have weaker formation ability than sh-NC cells. (D) Transwell experiment observed the changes in migration and invasion ability of OTUB2 knockdown cells compared to sh-NC cells. (E) Cell cycle distribution of OTUB2 knockdown cells and sh-NC cells were detected by flow cytometry.