Research Paper Volume 16, Issue 14 pp 11339—11358
PART1 facilitates tumorigenesis and inhibits ferroptosis by regulating the miR-490-3p/SLC7A11 axis in hepatocellular carcinoma
Figure 6. The knockdown of SLC7A11 inhibited motility and promoted apoptosis in HCC cells. (A–C) The migration and invasion were detected by Transwell assay (40 × objective lens) in HCCLM3 and Huh7 cells transfected with siRNA-SLC7A11.1 and -SLC7A11.3. The protein markers of the apoptosis were detected by Western blotting. (D) The expression of apoptosis and EMT-related proteins (E-cadherin, vimentin, and N-cadherin) was detected by Western blotting. (E) The relative levels of MDA and iron in HCCLM3 and Huh7 cells transfected with siRNA-SLC7A11.1 and -SLC7A11.3 in the erastin-induced ferroptosis assay. *p < 0.05, **p < 0.01, ***p < 0.001.