Research Paper Volume 16, Issue 13 pp 11103—11116

Figure 6. LDHA overexpression decreases the inhibitory effect of HOXC4 knockdown on cell proliferation and glycolysis. PC cells were divided into three groups, including normal control group (NC), HOXC4 knockdown group (sh-HOXC4) and cells with sh-HOXC4 and LDHA-overexpression. (A, B) Reverse transcription-quantitative PCR was used to detect the expression of LDHA in each group. (C) Cell count kit-8 assay was performed to detect the cell proliferation of each group. (D) Colony formation assay was performed to detect the colony formation of each group. (E) Analysis of the extracellular acidification rate for each group. (F, G) Glucose uptake and lactate production were analyzed in each group. ^*P < 0.05, ^**P < 0.01.