Research Paper Volume 16, Issue 14 pp 11208—11223

Figure 8. PI3K/AKT-mTOR pathway was related to the CCNL1-induced ADM resistance and progression of OS. (A) Western blot results of CCNL1, p-AKT, AKT, p-mTOR, mTOR, and GAPDH in HOS cells. (B) Western blot results of MRP1, Survivin, MMP2, and GAPDH in HOS cells. (C) qRT-PCR results of the related mRNAs CCNL1, AKT, mTOR, MRP1, Survivin, and MMP2 in HOS cells. ^*P < 0.05, vs. vector+DMSO; ^#P < 0.05, vs. CCNL1+DMSO; ^&P< 0.05, vs. vector+BKM120. (D) Wound-healing assay of HOS cells at 0 h, 24 h. Bar = 200 μm. ^*P < 0.05, ^**P < 0.01. (E) The rate of tunnel-positive HOS cells when exposing to ADM. Bar = 200 μm. ^**P < 0.01.