Research Paper Volume 16, Issue 14 pp 11208—11223

Figure 4. CCNL1 was associated with ADM resistance. The expression of MRP1 and P-gp was examined by western blot (A) and qRT-PCR analysis (B). ^**P < 0.01, vs. HOS; ^##P < 0.01, vs. 143B. (C) The expression level of CCNL1 was examined by western blot and qRT-PCR (D). ^**P < 0.01, vs. HOS; ^##P < 0.01, vs. 143B. (E) The viability of the os cells was accessed by MTT assay. ^*P < 0.05, ^#P < 0.05. The expression of CCNL1 was examined by western blot (F) and qRT-PCR analysis (G). ^***P < 0.001, ch-NC vs. sh-CCNL1. The viability of HOS/ADM (H) and 143B/ADM cells (I) was accessed by MTT assay. The rate of tunnel-positive cells increased in the sh-CCNL1 group in comparison to the NC group, bar = 200 μm (J).