Research Paper Advance Articles

Multi omics analysis of mitophagy subtypes and integration of machine learning for predicting immunotherapy responses in head and neck squamous cell carcinoma

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Figure 7. Knockdown of SLC26A9 promoted the proliferation and migration of HNSC cells. (A) SLC26A9 siRNA transfection levels analyzed via Western Blot. (B) CCK8 assay assessing cell viability in SCC15 and HN30 cells with reduced SLC26A9 expression. (C) EdU assay evaluating cell proliferation post SLC26A9 knockdown in SCC15 and HN30 cells. (D, E) Quantitative analysis of EdU-positive cell rates in SCC15 (D) and HN30 (E) cells. (F) Notable reduction in clone numbers in SCC15 and HN30 cells following SLC26A9 knockdown. (G) Transwell assay measuring migration capability of SCC15 and HN30 cells with decreased SLC26A9 expression. (H, I) Cell scratch assay examining proliferation in SCC15 (H) and HN30 (I) cells post SLC26A9 reduction. (J, K) Quantitative summary of clone numbers, migration rates in transwell assay, and wound healing rates in SCC15 (J) and HN30 (K) cells.