Research Paper Advance Articles
Comprehensive analysis of LMNB2 in pan-cancer and identification of its biological role in sarcoma
Figure 6. Down-regulation of LMNB2 inhibited cell proliferation by affecting cell cycle pathways. (A) RT-PCR assay was used to analyze LMNB2 expression levels in SARC tissues and normal tissues. (B) The inhibition efficiency of the two si-LMNB2 inhibitors was analyzed by RT-PCR. (C) MTT assay was performed to analyze cell proliferation. (D) Flow cytometry analysis was performed to analyze the cell cycle distribution. (E) Western blot assay was used to determine protein expression levels. (F) The association between LMNB2 expression levels and cyclinE1/cyclinD1 expression levels were explored by using the GEPIA2 database.