Figure 2. The therapy of Ns affected intervertebral disc degeneration in the intervertebral disc of CS rats. (A) H&E staining of cervical intervertebral disc in different groups 4 weeks after intervening, note: scale bar = 100 um. (B) Transmission electron microscope of the nucleus pulposus in different groups 4 weeks after intervening, note: scale bar = 2 um. (C) The neck-skin response thresholds to the mechanical stimuli applied by von Frey filaments in different groups 4 weeks after intervening. (D) Pfirrmann MRI Grades in different groups 4 weeks after intervening. Note: values are means ± SEMs, n = 5 per group, *p < 0.05, **p < 0.01.