Research Paper Advance Articles

Figure 4. Overexpression of NAT1 blocks PI3K/Akt/mTOR signaling pathway, thereby inhibiting EMT and glycolysis in colorectal cancer cells. (A) Transcriptome sequencing of showed that PI3K/AKt signaling pathway was enriched; (B) Overexpression of NAT1 significantly inhibited the protein expression of PI3K/Akt/mTOR signaling pathway; (C, D) Reactivation of mTOR can reduce the migration and invasion ability of colorectal cancer cells; (E, F) Reactivation of mTOR mitigated the inhibition of glycolysis by overexpression of NAT1; (G, H) Activation of PI3K/Akt/mTOR signaling pathway reversed the inhibitory effect of NAT1 overexpression on EMT (N-cadherin, Vimentin) and glycolytic (GLUT1, LDHA) related proteins.