Research Paper Advance Articles

Figure 3. Overexpression of NAT1 inhibits EMT and glycolysis in colorectal cancer cells. (A) Snail expression in TCGA database was negatively correlated with NAT1; (B) Immunofluorescence results showed that overexpression of NAT1 could promote the expression of E-cadherin (P < 0.05); (C, D) Overexpression of NAT1 significantly inhibited the expression of EMT-related proteins (N-cadherin, Snail and Vimentin), and promoted the expression of E-cadherin (P < 0.05); (E) Overexpression of NAT1 significantly inhibited the glucose uptake ability of colorectal cancer cells; (F) ECAR assay showed that NAT1 significantly inhibited the glycolysis and storage capacity of colorectal cancer; (G) GLUT1 was negatively correlated with NAT1 expression in TCGA database. (H, I) Overexpression of NAT1 inhibited the expression of glycolytic related metabolic enzymes (GLUT1, LDHA).