Figure 2.Overexpression of NAT1 inhibits proliferation, migration and liver metastasis of colorectal cancer cells. (A) NAT1 was highly expressed in HCT116 cell line and low expressed in SW480 cell line; (B, C) NAT1 was knocked down in HCT116 cell line and was overexpressed in SW480 cell line; (D, E) Scratch test showed that overexpression of NAT1 could inhibit cell migration ability (P < 0.05); (F) Transwell assay showed that overexpression of NAT1 significantly inhibited cell invasion (P < 0.05); (G) Plate cloning results showed that high expression of NAT1 had inhibitory effect on cell proliferation (P < 0.05); (H) CCK8 assay also confirmed that NAT1 significantly inhibited cell proliferation (P < 0.05); (I–K) In vitro animal experiments confirmed that overexpression of NAT1 can inhibit the liver metastasis ability of colorectal cancer cells.
