Research Paper Advance Articles

Figure 2. Overexpression of NAT1 inhibits proliferation, migration and liver metastasis of colorectal cancer cells. (A) NAT1 was highly expressed in HCT116 cell line and low expressed in SW480 cell line; (B, C) NAT1 was knocked down in HCT116 cell line and was overexpressed in SW480 cell line; (D, E) Scratch test showed that overexpression of NAT1 could inhibit cell migration ability (P < 0.05); (F) Transwell assay showed that overexpression of NAT1 significantly inhibited cell invasion (P < 0.05); (G) Plate cloning results showed that high expression of NAT1 had inhibitory effect on cell proliferation (P < 0.05); (H) CCK8 assay also confirmed that NAT1 significantly inhibited cell proliferation (P < 0.05); (I–K) In vitro animal experiments confirmed that overexpression of NAT1 can inhibit the liver metastasis ability of colorectal cancer cells.