Research Paper Volume 16, Issue 12 pp 10462—10476
MATN4 as a target gene of HIF-1α promotes the proliferation and metastasis of osteosarcoma
Figure 6. Knockdown of HIF-1α reduced the promoting effects on proliferation of OS cell induced by MATN4 overexpression. (A) Protein expression of HIF-1α and MATN4 were detected by western blotting in each group cells under hypoxia. (B, C) CCK-8 and EDU assays were used to detect the proliferation ability in each group cells under hypoxia. (D) Colony formation assays were used to detect the colony formation ability in each group cells under hypoxia. *, p <.05; **, p <.01.