Research Paper Volume 16, Issue 12 pp 10462—10476
MATN4 as a target gene of HIF-1α promotes the proliferation and metastasis of osteosarcoma
Figure 5. MATN4 was co-expressed with HIF-1α and regulated as a target gene of HIF-1α in OS cells and tissue. (A) Motif of HIF-1α. (B) Potential binding site of HIF-1α in the MATN4 promoter area. (C) CHIP and RT-qPCR assay were performed to verify that HIF-1α bound and up-regulated expression of MATN4 under hypoxia in vitro. (D, E) RT-qPCR and western blotting were performed to detect mRNA and protein expression of MATN4 in 143B and HOS cells transfected with sh-HIF1α or Lv-HIF1α under hypoxia. (F) Pearson correlation analysis was performed to demonstrate co-expression of HIF-1α and MATN4. (G) IHC assays were performed to show co-expression HIF-1α and MATN4 in vivo. *, p <.05; **, p <.01.