Research Paper Advance Articles
MATN4 as a target gene of HIF-1α promotes the proliferation and metastasis of osteosarcoma
Figure 3. Inhibition of MATN4 significantly reversed the promoting effects of hypoxia on the proliferation ability of OS cells. (A, B) RT-qPCR and western blotting were used to detect mRNA and protein expression of MATN4 in OS cells transfected with sh-scramble or SH-MATN4 under normoxia and hypoxia. (C, D) CCK-8 and EDU assays were used to detect the proliferation ability of OS cells transfected with sh-scramble or SH-MATN4 at 24h and 48h under normoxia and hypoxia. (E) Colony formation assays were used to detect the colony formation ability of OS cells transfected with sh-scramble or SH-MATN4 at 24h and 48h under normoxia and hypoxia. *, p <.05; **, p <.01.