Research Paper Volume 16, Issue 12 pp 10462—10476
MATN4 as a target gene of HIF-1α promotes the proliferation and metastasis of osteosarcoma
Figure 2. MATN4 knockdown significantly inhibited proliferation, migration and invasion abilities of OS cells under normoxia. (A, B) Confirmation of MATN4 knockdown by RT-qPCR and western blotting in 143B and HOS cells. (C, D) CCK-8 and EDU staining assays were performed to detect the proliferation ability of sh-scramble and SH-MATN4 OS cells at 24h and 48h. (E) Colony formation assays were performed in sh-scramble and SH-MATN4 OS cells. (F) Transwell assays were performed to detect invasion ability of OS cells after MATN4 knockdown. (G) Wound healing assays were used to detect migration ability of OS cells after MATN4 knockdown. *, p <.05; **, p <.01.