Research Paper Advance Articles
MATN4 as a target gene of HIF-1α promotes the proliferation and metastasis of osteosarcoma
Figure 1. MATN4 was involved in OS cell metastasis. (A) Volcano plots were used to exhibit the differentially expressed genes (DEGs) related to metastasis and hypoxia, based on gene expression profiles obtained from the Target database. (B) An intersection of DEGs: TP53, CA9, and MATN4. (C) Expression levels of TP53, CA9 and MATN4 in 143B and HOS cells were measured by RT-qPCR under normoxia or hypoxia. (D) IHC was used to exhibit protein expression level of MATN4 in OS tissue and non-cancerous bone tissue. (E, F) Western blotting and RT-qPCR to measure MATN4 mRNA and protein expression under varying hypoxic conditions. (G, H) Western blotting and RT-qPCR to measure the MATN4 protein levels in normal cells (hFOB1.19 and BMSC), in OS cells with low-metastatic ability (MG63, U2OS, Saos2, HOS), and OS cells with strong metastatic ability (MNNG/HOS, SJSA-1, 143B). *, p <.05; **, p <.01.