Research Paper Advance Articles

Figure 4. NGR1 inhibits breast cancer cell proliferation by down-regulating RUNX2 protein expression. (A) After transfection of overexpression RUNX2 plasmid (2 μg/mL) in SK-BR-3 cells for 48 h, the expression of RUNX2 protein and mRNA was detected by Western blot and QPCR assay. (B) After transfection of siRNA (100 nmol/L) in MDA-MB-231 cells for 48 h, the expression of RUNX2 protein and mRNA expression. (C) Breast cancer cells were transfected with overexpression plasmid and siRNA for 48 h of incubation, followed by NGR1 treatment for 24 h, 48 h, and 72 h. Changes in cell viability were detected by CCK8 assay. (D) Expression levels of RUNX2 mRNA were detected by QPCR assay to examine the expression levels of RUNX2 mRNA in breast cancer cell lines. (^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, ^**** P < 0.0001; ^# P < 0.0001, ^## P < 0.0001, ^### P < 0.0001, ^#### P < 0.0001).