Research Paper Advance Articles
Notoginsenoside R1 (NGR1) regulates the AGE-RAGE signaling pathway by inhibiting RUNX2 expression to accelerate ferroptosis in breast cancer cells
Figure 3. NGR1 inhibits breast cancer cell viability and RUNX2 expression. (A) The expression levels of RUNX2 protein and mRNA in HBL-100 and different breast cancer cell lines were detected by Western blot assay. (B) Changes in cell viability were detected by CCK8 assay after different concentrations of NGR1 (25, 50, 100, 200, 400 μmol/L) were treated with SK-BR-3 and MDA-MB-231 cells for 24 h, 48 h, and 72 h, respectively. (C) Western blot detection of RUNX2 protein after treatment of MDA-MB-231 cells with different concentrations of NGR1 (25, 50, 100 μmol/L) for 48 h. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).