Research Paper Volume 16, Issue 11 pp 9334—9349

Figure 6. Cell cycle progression is elevated in BNIP3(L)-high MCF7 cells. MCF7 cells stably-transduced with the BNIP3(L)-GFP constructs were subjected to FACS sorting, to isolate the 5% highest GFP (GFP-high) and the 5% lowest GFP (GFP-low) sub-populations. Cell cycle progression was evaluated with propidium iodide by flow cytometry. The percentage of cells in G0/G1, S, and G2/M phases of the cell cycle are represented in the pie graphs. Data are shown as the mean ± standard deviation (SD) (n = 3). Statistical significance was determined using an unpaired Student’s t-test, *p ≤ 0.05, **p ≤ 0.01.