Research Paper Volume 16, Issue 11 pp 9334—9349

Figure 5. Mitochondrial respiration and glycolysis are significantly enhanced in BNIP3(L)-high MCF7 cells. MCF7 cells stably-transduced with the BNIP3(L)-GFP constructs were subjected to FACS sorting, to isolate the 5% highest GFP (GFP-high) and the 5% lowest GFP (GFP-low) sub-populations. The Seahorse XF96 analyser was employed to assess their mitochondrial and glycolytic function. Mitochondrial function results for BNIP3-GFP cells (A) and BNIP3L-GFP (B) are shown as an OCR tracing in left panel, and bar graphs for proton leak, ATP production, basal respiration, spare respiratory capacity and maximal respiration, obtained from the OCR quantification. Data are shown as the mean ± standard deviation (SD) (n = 5). Statistical significance was determined using an unpaired Student’s t-test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. not statistically significant. Glycolytic function results for BNIP3-GFP cells (C) and BNIP3L-GFP (D) are shown as an ECAR tracing in left panel, and bar graphs for glycolysis, glycolytic capacity and glycolytic reserve, obtained from the ECAR quantification. Data are shown as the mean ± standard deviation (SD) (n = 5). Statistical significance was determined using an unpaired Student’s t-test, * p ≤ 0.05, ** p ≤ 0.01, n.s. not statistically significant.