Research Paper Volume 16, Issue 12 pp 10348—10365

Figure 1. Single-cell transcriptional analysis of T lymphocytes from LUAD patient biopsies. (A) Schematic showing the analysis of procedures used for this study (created using BioRender.com). (B) Bar plot showing the cell number (Log₁₀ transformed) of samples selected from primary patients’ data (ut: untreatment; tr: treatment; meta: lymph node or liver metastasis; pri: primary tumor). (C) Bar plot showing the cell number (Log₁₀ transformed) of pre-PD-1 blockade therapy and post-PD-1 blockade therapy. (D) Bar plot showing the cell number (Log₁₀ transformed) of groups which were defined by biopsy sites (metastasis or primary tumor) and response of PD-1 blockade therapy (good or poor response). (E) Two-dimensional UMAP plot of single-cell RNA-Seq (scRNA-Seq) performed on groups A, B, and C after PD-1 blockade therapy (horizontal axis: UMAP_1, vertical axis: UMAP_2). (F) Patient group preference for each CD4 and CDD8 subcluster measured using the RO/E index (dark blue: enrichment, light blue: depletion).