Research Paper Volume 16, Issue 13 pp 10813—10831
An immune biomarker associated with EMT serves as a predictor for prognosis and drug response in bladder cancer
Figure 4. Cell dynamics in the progression of bladder cancer. (A, B) tSNE and UMAP cell clustering analysis to distinguish cell types in the tumor microenvironment. (C, D) Tumor cells showing WDHD1 expression sites. (D) epithelial cells were re-clustered using tSNE and UMAP. (E) Changes of cell types and WDHD1 expression with pseudotime analysis. (F) Western blot showed that decreased expression of WDHD1 inhibited the expression of N-Cadherin,Vimentin, phosphorylated focal adhesion kinase (p-FAK) .The tubulin was used as loading control. *p < 0.05, **p < 0.01