Research Paper Advance Articles
MiR-33a-5p in stored red blood cells regulates genes of innate immune response and promotes inflammation
Figure 4. (A) The net plot of mir-33a-5p and its regulated mRNA target genes. (B) The signaling pathway of KEGG showed that the target mRNA genes were enriched in the adipocytokine signaling pathway and the PPARα/AMPK/CPT-1a pathway was the key signaling pathways. (C) qRT–PCR to examine miR-33a-5p expression. (*P<0.05 vs NC, #P<0.05 vs Mimic). (D) qRT–PCR to examine PPARα/ACC2/AMPK/CPT-1a mRNA expression. (*P<0.05 vs NC, #P<0.05 vs Mimic). NC: normal macrophages group; Mimic-NC: macrophages transfected with the mimic negative control; Mimic: macrophages transfected with the miR-33a-5p mimic; Int: macrophages transfected with the inhibitor; Int-NC:macrophages transfected with the negative inhibitor control. All data are means ± SD; n = 3.