Research Paper Volume 16, Issue 12 pp 10239—10251

MiR-33a-5p in stored red blood cells regulates genes of innate immune response and promotes inflammation

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Figure 3. (A) Flow cytometric examination of CD86/NOS2 expression of macrophages. (B) qRT–PCR for examination of PPARα/ACC2/AMPK/CPT-1a mRNA expression (*P<0.05). (C) Protein concentrations of IL-1β/IL-6/IL-12/TNF-α in the media of cells from different groups (*P<0.05). NC: normal macrophages group; Mimic: macrophages transfected with the miR-33a-5p mimic. All data are means ± SD; n = 3.