Research Paper Volume 16, Issue 12 pp 10216—10238

Figure 7. NR4A3 is necessary for EGR1-induced apoptosis and the impairment of ECM synthesis metabolism in human NPCs in vitro. (A) NPCs were transfected with EGR1 overexpression plasmids and NR4A3 siRNA, followed by flow cytometry analysis to determine the percentage of apoptotic cells in the different groups. (B) Quantitative analysis of the NPC apoptosis rate. (C) Western blot analysis was performed to determine the protein expression levels of NR4A3, Bax, Bcl-2, caspase-3, aggrecan, and collagen II in the different treatment groups. (D–I) Quantitative analysis of the NR4A3, Bax, Bcl-2, caspase-3, aggrecan, and collagen II protein levels. The data are expressed as the mean ± SD (n = 3). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.