Research Paper Volume 16, Issue 11 pp 9846—9858
LncRNA MIR181A2HG inhibits keratinocytes proliferation through miR-223-3p/SOX6 axis
Figure 6. The MIR181A2HG/miR-223-3p/SOX6 axis regulated the proliferation of HaCaT keratinocytes. (A) CCK-8 kit was applied to detect the cell viability of HaCaT keratinocytes co-transfected with NC inhibitor/miR-223-3p inhibitor and si-NC/si-SOX6. (B, C) qRT-PCR and Western blotting were performed to detect the KRT6/KRT16 level in HaCaT keratinocytes co-transfected with NC inhibitor/miR-223-3p inhibitor and si-NC/si-SOX6. (D) CCK-8 kit was applied to detect the cell viability of HaCaT keratinocytes co-transfected with pcDNA3.1/pcDNA3.1-MIR181A2HG and NC mimic/miR-223-3p mimic. (E, F) qRT-PCR and Western blotting were performed to detect the KRT6/KRT16 level in HaCaT keratinocytes co-transfected with pcDNA3.1/pcDNA3.1-MIR181A2HG and NC mimic/miR-223-3p mimic. GAPDH served as an internal reference. *P<0.05, **P<0.01, ***P<0.001.