Research Paper Volume 16, Issue 11 pp 9846—9858

Figure 5. The modulation of MIR181A2HG could affect SOX6 expression. (A) Expression correlation of MIR181A2HG and SOX6 in datasets (GSE13355, GSE14905 and GSE50790). (B, C) HaCaT keratinocytes transfected for 48 hours were harvested and qRT-PCR/Western blotting was used to detect the expression of SOX6 in HaCaT keratinocytes with the transfection of si-MIR181A2HG/pcDNA3.1-MIR181A2HG. (D) CCK-8 kit was applied to detect the cell viability of HaCaT keratinocytes co-transfected with pcDNA3.1/pcDNA3.1-MIR181A2HG and si-NC/si-SOX6. (E, F) qRT-PCR and Western blotting were performed to detect the KRT6/KRT16 level in HaCaT keratinocytes co-transfected with pcDNA3.1/pcDNA3.1-MIR181A2HG and si-NC/si-SOX6. GAPDH served as an internal reference. *P<0.05, **P<0.01, ***P<0.001.