Research Paper Volume 16, Issue 11 pp 9813—9823
Targeting cGAS-STING signaling protects retinal ganglion cells from DNA damage-induced cell loss and promotes visual recovery in glaucoma
Figure 4. Lipid oxidation modulates the activation of cGAS-STING induced by cisplatin. (A) Lipid oxidation was detected when RGC-5 cells were treated with cisplatin followed by LX-1. (B) mRNA expression of cGAS and STING was detected by real-time PCR when RGC-5 cells were treated with cisplatin followed by LX-1. (C) Protein expression of cGAS and STING was detected by western blot when RGC-5 cells were treated with cisplatin followed by LX-1. (D) mRNA expression of Il6, Ifnb, and Cxcl10 was detected by real-time PCR when RGC-5 cells were treated with cisplatin followed by LX-1. (E) The mRNA expression of cGAS, STING, Il6, Ifnb, and Cxcl10 was detected by real-time PCR in RGC-5 cells that were treated with cisplatin followed by NAC. *p<0.05.