Research Paper Volume 16, Issue 11 pp 9813—9823
Targeting cGAS-STING signaling protects retinal ganglion cells from DNA damage-induced cell loss and promotes visual recovery in glaucoma
Figure 3. Inflammatory response to cGAS-STING activation in glaucoma. (A) The mRNA expression of Il6, Ifnb, and Cxcl10 in glaucoma was detected by real-time PCR. (B) ELISA analysis was conducted to measure the level of IL-6, IFN-β, and CXCL10 in glaucoma. (C) mRNA expression of Il6, Ifnb, and Cxcl10 in RGC-5 cells treated with cisplatin, followed by C176 was detected by real-time PCR. (D) The cytokine level of Il6, Ifnb, and Cxcl10 in RGC-5 cells treated with cisplatin, followed by C176 was detected by ELISA. (E) Genetic inhibition of cGAS-STING combined with cisplatin on the expression of Il6, Ifnb, and Cxcl10 in RGC-5 cells was detected by real-time PCR. *p<0.05.