Research Paper Volume 16, Issue 11 pp 9813—9823
Targeting cGAS-STING signaling protects retinal ganglion cells from DNA damage-induced cell loss and promotes visual recovery in glaucoma
Figure 2. cGAS-STING activation occurred along with RGC apoptosis in DNA damage. (A) The mRNA expression of cGAS and STING in the glaucoma retina was detected by real-time PCR. (B) The expression of cGAS and STING in the glaucoma retina was detected by immunofluorescence. (C) The mRNA expression of cGAS and STING in cell line RGC-5 after being treated with cisplatin was detected by real-time PCR. (D) Cell apoptosis was detected by flow cytometry after RGC-5 cells were treated with C176 and cisplatin. (E) Cell apoptosis was detected by flow cytometry after RGC-5 cells were treated with C176 (siSTING) combined with cisplatin. *p<0.05.